The determination of ammonia and urea in blood

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Determination of ammonia and urea in urine and of urea in blood by use of an ammonia-selective electrode.

I propose a single, quick method for measuring ammonia in urine and urea in plasma and urine. An ammonia-selective electrodie is used, set up on a microcell, which allows use of small sample volumes. Ammonia is measured directly after partial conversion of ammonium ions to NH3. Urea is measured after its hydrolysis by urease. With urine, the two procedures can be carried out successively in the...

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New Methods for the Determination of Total Non-protein Nitrogen, Urea and Ammonia in Blood

The analytical technique described in the preceding three papers lends itself peculiarly well to the determination of total uncoagulable nitrogen, urea and ammonia in blood, milk, eggs and other liquids where we are dealing with minute amounts of these different constituents. In two earlier papers results obtained by adaptations of these methods to blood analysis were published.’ The procedures...

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New Methods for the Determination of Total Non-protein Nitrogen, Urea and Ammonia in Blood

The analytical technique described in the preceding three papers lends itself peculiarly well to the determination of total uncoagulable nitrogen, urea and ammonia in blood, milk, eggs and other liquids where we are dealing with minute amounts of these different constituents. In two earlier papers results obtained by adaptations of these methods to blood analysis were published.’ The procedures...

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The Determination of Blood Urea

Most of the methods for the determination of urea in blood involve the use of the enzyme urease to hydrolyze the urea, since this reaction is specific. However, instead of crystalline urease (l), crude jack bean meal extracts are used. These extracts have been found to contain other enzymes besides urease, termed “ammonia-producing” enzymes, and their substrates, which may produce large quantit...

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The Determination of Blood Urea

Most of the methods for the determination of urea in blood involve the use of the enzyme urease to hydrolyze the urea, since this reaction is specific. However, instead of crystalline urease (l), crude jack bean meal extracts are used. These extracts have been found to contain other enzymes besides urease, termed “ammonia-producing” enzymes, and their substrates, which may produce large quantit...

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ژورنال

عنوان ژورنال: Experimental Biology and Medicine

سال: 1906

ISSN: 1535-3702,1535-3699

DOI: 10.3181/00379727-4-61